Plasmid_Backbone

Part:BBa_K1137006:Design

Designed by: Matthew Deyell   Group: iGEM13_Paris_Bettencourt   (2013-10-04)


pSB6A3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3544
    Illegal suffix found at 4635
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3544
    Illegal SpeI site found at 4636
    Illegal PstI site found at 4650
    Illegal NotI site found at 3550
    Illegal NotI site found at 4643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3544
    Illegal XhoI site found at 3420
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3544
    Illegal suffix found at 4636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3544
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3559
    Illegal SpeI site found at 4636
    Illegal PstI site found at 4650
    Illegal NgoMIV site found at 189
    Illegal AgeI site found at 4346
    Illegal AgeI site found at 4458
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 774
    Illegal SapI.rc site found at 2434


Design Notes

Designed to be stable with pSB9S1, pSB10K1, and pSB3C7


Source

Original sequence used was pSB1C3, replacing the ori and resistance cassette with those from pET

References